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51.Title: |
EXTRACTION AND PURIFICATION OF PROTEINS FROM E. coli WITHOUT HARVESTING CELLS
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Authors: |
Grabski, Anthony ; Drott, Don ; Handley, Mark ; Mehler, Mark ; Novy, Robert |
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Journal: |
TheScientificWorldJOURNAL |
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Page Range: |
36-38 |
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Date of publication: |
2/01/2002 |
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Article Type: |
Short Report |
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Domains: |
Gene, Molecular & Cell Therapy
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Abstract |
Extraction and separation of foreign proteins expressed in E. coli commonly employs multistep fractionation procedures combining centrifugation, mechanical disruption, volume reduction, filtration, and finally capture and recovery in order ...
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52.Title: |
FUNCTIONAL GENOMICS WITH YBL036c
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Authors: |
Berenfeld, Ludmilla ; Sokolova, Olga ; Rim, Sun Hee ; Ringe, Dagmar |
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Journal: |
TheScientificWorldJOURNAL |
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Page Range: |
35-35 |
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Date of publication: |
2/01/2002 |
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Article Type: |
Short Report |
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Domains: |
Gene, Molecular & Cell Therapy
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Abstract |
Functions of more than 2,000 genes in the Saccharomyces cerevisiae genome are still unknown. Gene YBL036c is among them. Gene YBL036c encodes a protein that is PLP (pyridoxal-phosphate) dependent. Its sequence does not indicate similarity t ...
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53.Title: |
AUTOMATED PROTEIN PURIFICATION FOR STRUCTURAL STUDIES
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Authors: |
Andersson, Hans O.; Eklund, Par ; Galin, Markus ; Hedqvist, Lotta ; Liljedahl, Pia ; Markeland, Christine ; Pless, Thomas ; Sigrell, Jill A.; Torstenson, Karin |
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Journal: |
TheScientificWorldJOURNAL |
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Page Range: |
33-34 |
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Date of publication: |
2/01/2002 |
| |
Article Type: |
Short Report |
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Domains: |
Gene, Molecular & Cell Therapy
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Abstract |
Structural genomics, also known as structural proteomics, aims to resolve protein functions on a large scale[1]. To determine structures using either X-ray crystallography or NMR, fairly large amounts (>10 mg) of target proteins (which are ...
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54.Title: |
STRUCTURAL PROTEOMICS OF EUKARYOTIC GENE FAMILIES
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Authors: |
Montelione, Gaetano T.; Anderson, Stephen ; Acton, Thomas ; Dixon, Bonnie ; Huang, Yuanpeng ; Moseley, Hunter ; Monleon, Daniel ; Shukla, Kamal ; Swapna, Gaetano G.V.T.; Tejero, Roberto |
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Journal: |
TheScientificWorldJOURNAL |
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Page Range: |
32-32 |
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Date of publication: |
2/01/2002 |
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Article Type: |
Short Report |
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Domains: |
Gene, Molecular & Cell Therapy
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Abstract |
Genome sequencing projects have already determined nearly complete genome sequences of several organisms, including human. The products of these genes are widely recognized as the next generation of therapeutics and targets for the developm ...
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55.Title: |
PROGRESS IN AB INITIO PROTEIN STRUCTURE PREDICTION
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Authors: |
Baker, David |
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Journal: |
TheScientificWorldJOURNAL |
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Page Range: |
31-31 |
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Date of publication: |
2/01/2002 |
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Article Type: |
Short Report |
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Domains: |
Gene, Molecular & Cell Therapy
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Abstract |
I will discuss the application of the Rosetta method for ab nitio protein structure prediction to the prediction of protein structure and function on the genome scale. Rosetta is based on a picture of protein folding in which short segments ...
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56.Title: |
PATTERN OF ORGANIZATION OF HUMAN MITOCHONDRIAL PSEUDOGENES IN THE NUCLEAR CHROMOSOMES
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Authors: |
Woischnik, Markus ; Moraes, Carlos T. |
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Journal: |
TheScientificWorldJOURNAL |
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Page Range: |
27-30 |
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Date of publication: |
2/01/2002 |
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Article Type: |
Short Report |
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Domains: |
Gene, Molecular & Cell Therapy
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Abstract |
Mitochondrial pseudogenes in the human nuclear genome have been previously described, mostly as a source of artifacts during the analysis of the mitochondrial genome. With the availability of the complete human genome sequence, we performed ...
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57.Title: |
INTEINS IN MICROBIAL GENOMES: DISTRIBUTION, MECHANISM, AND FUNCTION
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Authors: |
Southworth, Maurice W.; Perler, Francine B. |
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Journal: |
TheScientificWorldJOURNAL |
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Page Range: |
25-26 |
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Date of publication: |
2/01/2002 |
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Article Type: |
Short Report |
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Domains: |
Gene, Molecular & Cell Therapy
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Abstract |
Inteins are self-splicing protein elements (134 to 608 amino acids). Over 125 inteins have been cataloged in InBase, the on-line intein database (http://www.neb.com/neb/inteins.html), which includes the Intein Registry[1]. Inteins naturally ...
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58.Title: |
PROTEOMIC ANALYSIS OF ROUGH AND SMOOTH ENDOPLASMIC RETICULUM
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Authors: |
Paiement, Jacques ; Roy, Line ; Gilchrist, Annalyn ; Bell, Alex ; Kearney, Rob ; Thomas, David Y.; Bergeron, John J.M. |
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Journal: |
TheScientificWorldJOURNAL |
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Page Range: |
23-24 |
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Date of publication: |
2/01/2002 |
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Article Type: |
Short Report |
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Domains: |
Gene, Molecular & Cell Therapy
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Abstract |
The membrane subcompartments of the endoplasmic reticulum (ER) are known to be continuous but yet complete structural and functional identity is maintained in each. This is achieved by membrane differentiation and maturation and likely invo ...
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59.Title: |
COMPARING THE SPEED AND ACCURACY OF THE SMITH AND WATERMAN ALGORITHM AS IMPLEMENTED BY MPSRCH WITH THE BLAST AND FASTA HEURISTICS FOR SEQUENCE SIMILARITY SEARCHING
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Authors: |
Muratet, Michael A. |
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Journal: |
TheScientificWorldJOURNAL |
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Page Range: |
21-22 |
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Date of publication: |
2/01/2002 |
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Article Type: |
Short Report |
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Domains: |
Gene, Molecular & Cell Therapy
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Abstract |
Similarity searching is used to identify homologies between a query sequence and sequences in a database to elucidate the function of the former by considering the latter. Similarity searching (or more appropriately, dissimilarity searching ...
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60.Title: |
CANDIDATE GENE IN PREDICTING IN VIVO OVARIAN CANCER RESPONSE TO COMBINATION THERAPY WITH PARAPLATIN AND PACLITAXEL
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Authors: |
Mirhashemi, Ramin ; Arena, J. Fernando; Frudakis, Tony ; Lambrou, Nicholas ; Arboleda, Jane ; Hunt, Marsha ; Medranda, Maria ; Averette, Hervy ; Penalver, Manuel |
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Journal: |
TheScientificWorldJOURNAL |
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Page Range: |
19-20 |
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Date of publication: |
2/01/2002 |
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Article Type: |
Short Report |
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Domains: |
Gene, Molecular & Cell Therapy
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Abstract |
Adverse drug reactions, due at least in part to interindividual variability in drug response, rank between the 4th and 6th leading causes of death in the U.S. The field of “pharmacogenetics” (the study of variability in drug response due to ...
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