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Title: |
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Electrochemical Study of Biotin-Modified Self-Assembled Monolayers: Recommendations for Robust Preparation |
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Authors: |
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Brown, Richard J.C.; Brett, Dan J.L. |
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Journal: |
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TheScientificWorldJOURNAL |
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Year: |
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2006 |
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Volume: |
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6 |
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Page Range: |
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20-29 |
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Article Type: |
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Research Article |
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Domains: |
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Environmental Chemistry
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Analytical Chemistry
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DOI: |
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10.1100/tsw.2006.20 |
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Synopsis: |
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Many applications in biochemistry and biotechnology require chemically functionalised surfaces that will be receptive to specific biological compounds. The first step in this process is to form a monolayer of suitable linking compounds on a substrate. This paper describes work to measure and optimise electrochemically the formation and packing of self-assembled monolayers (SAMs) of thiol-terminated molecules on a gold surface, and introduces the novel concept of “equivalent alkane thiol chain length” to describe the layers’ characteristics. |
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Keywords: |
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self-assembly monolayers, biotin functionalisation, impedance spectroscopy |
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Abstract |
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The development of the underpinning methodology for the production of robust, well-formed, and densely packed biotin-HPDP functionalised gold surfaces, the crucial first step in immobilising bimolecules on surfaces, is described.
Self-assembled monolayers (SAMs) with biotin end-groups were prepared on polycrystalline gold surfaces according to a published method. The layers formed were studied using cyclic voltammetry to determine the composition of the layer and its quality. Crystal impedance spectroscopy was also applied as a complimentary indicator of the composition of the layer.
For the first time, the effect of assembly time on the properties of the layer was studied along with the composition of the layer and the ability of the precursor molecule to self-assemble by oxidative addition. |
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